Data Center: Neuroscience

Astrocyte response to beta amyloid peptide 25-35

Reference:
Data available from GEO, dataset GDS519.

Time course analysis of cortical type I astrocytes cultured on beta amyloid peptide 25-35 fragment (bAP(25-35)). Samples examined at 12 hours, 1 day, 3 days, and 5 days. Amyloid plaques of Alzheimer's disease are composed of beta amyloid.

Abstract:
Alzheimer's disease is one of the most devastating neurological disorders. Recently, research into the mechanisms of this neurodegenerative disease have begun to provide new insights. In this study, cortical type-I astrocytes were cultured on beta-amyloid peptide fragment 25-35 for 12 hours, 1, 3, and 5 days. These samples were harvested at the indicated time, and hybridized to the Affymetrix U34 Neuro GeneChip. These arrays contain ~1300 genes specifically of interest in neuroscience research. Data was loaded and analyzed with GeneSifter, a microarray analysis system. Initial filtering (to remove genes not significantly expresed) reduced the gene list to 173 significant genes. Of this subset, the data clearly separated into two clusters, differing primarily at the 12 hour time point. Further analysis indicated that genes involved in signal transduction, including several kinases and their target transcription factors, were highly overrepresented at 12 hours.

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