Data Center: Cardiovascular

Transcriptome analysis of apoE -/- mouse aortas

Microarray Platform:
Affymetrix® GeneChip® Mouse Genome 430 2.0 array

Reference:
Data available from Gene Expression Omnibus GEO, GSE2372
GEO Summary: Aortas of 32 weeks old apoE -/- mice versus wild type mice on a C57BL/6J background.

Abstract:
Gene expression in aortas of 32 week old C57BL/6J apoE-/- mice was compared to that in wild type C57BL/6J mice using the Affymetrix® GeneChip® Mouse Genome 430 2.0 array. Using filtering criteria of a 1.5 or greater fold-change in expression, all "present calls" in at least one group and a false discovery rate of less than 5%, 861 out of over 39,000 transcripts were differentially expressed. The expression of 742 transcripts was increased in the apoE -/- aortas, while 119 showed decreased expression. The Gene Ontology and KEGG pathway terms associated with these genes were examined to identify biological themes associated with each set of genes. These analyses identified distinct biological themes associated with each group of genes. The ontology term analysis identified an over representation of genes involved in the immune response, cell adhesion and apoptosis among the up-regulated genes. The analysis of KEGG pathway terms identified a significant over representation of genes involved in the Jak-Stat signaling pathway and cytokine-cytokine receptor interactions among the up-regulated genes.

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